Journal: Molecular and Cellular Biology

Article Title: Deficiency of a Lipid Droplet Protein, Perilipin 5, Suppresses Myocardial Lipid Accumulation, Thereby Preventing Type 1 Diabetes-Induced Heart Malfunction

doi: 10.1128/MCB.00133-14

Figure Lengend Snippet: Levels of Plin and lipase proteins in the heart. (A) Images of immunoblotting of Plin proteins and lipases using total protein extracts from the heart (15 μg of total protein per lane). Coomassie brilliant blue (CBB) staining of the gel was used as a loading control. (B to F) The graphs show the levels of Plin5, Plin2, Plin3, ATGL, and HSL relative to those in saline-treated control WT mice (n = 3 per group). Open bars, WT mice; filled bars, Plin5−/− mice; ND, not detectable. Data are shown as means ± SEM (*, P < 0.05; **, P < 0.01; and ***, P < 0.001).

Article Snippet: Proteins were probed using the following antibodies: Plin5 (developed in-house [ 8 ] and by Progen [GP31]); Plin2 (GP40; Progen); Plin3 (3883; Prosci); ATGL (2138), phospho-p38 (4511), and p38 (9212) (Cell signaling); pan-cadherin (C3678), PKC (P5704), and p47 phox (SAB4502810) (Sigma); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (sc-25778) and β-tubulin (sc-9104) (Santa Cruz); hormone-sensitive lipase (HSL) (ab45422) and uncoupling protein 3 (UCP3) (ab3477) (Abcam); p67 phox (610912; BD Bioscience); and acyl coenzyme A (CoA) oxidase (Acox), very long-chain acyl-CoA dehydrogenase (VLCAD), and medium-chain acyl-CoA dehydrogenase (MCAD) (gifts from T. Hashimoto and S. Yamaguchi).

Techniques: Western Blot, Staining, Control, Saline

Journal: Journal of Lipid Research

Article Title: PNPLA2 influences secretion of triglyceride-rich lipoproteins by human hepatoma cells

doi: 10.1194/jlr.m090928

Figure Lengend Snippet: Figure 5A, no evidence was found that a combination of the two PNPLA2 inhibition methods leads to

Article Snippet: The cells were subsequently incubated for one hour with 1:50 diluted PNPLA2 monoclonal antibody (Novus Biologicals) conjugated with Alexa Fluor 488 using the Zenon Alexa Fluor 488 Mouse IgG2b Labeling Kit (Thermo Fisher Scientific) at RT, followed by overnight incubation with either 1:100 diluted PDI (Enzo) or 1:100 diluted PLIN2 (R&D Systems) monoclonal antibodies.

Techniques: Inhibition

Journal: The Journal of Biological Chemistry

Article Title: Defining a mechanistic link between pigment epithelium–derived factor, docosahexaenoic acid, and corneal nerve regeneration

doi: 10.1074/jbc.M117.801472

Figure Lengend Snippet: Concentration of compounds used in different treatments PEDF was purchased from Bio Products (Middletown, MD). DHA and atglistatin (a PEDF-R inhibitor) were purchased from Cayman Chemical (Ann Arbor, MI). The 44-mer PEDF peptide was prepared by GenWay Biotech, Inc. (San Diego). NPD1 was a kind gift from Dr. Nicos A. Petasis, Loker Hydrocarbon Research Institute, University of Southern California, Los Angeles.

Article Snippet: Eight-μm serial sections were washed with PBS (three times for 5 min), blocked with 10% normal goat serum plus 0.5% Triton X-100 solution in PBS for 1 h, and then incubated at 4 °C overnight with primary rabbit anti-PEDF-R antibody (Cayman, Ann Arbor, MI; 1:250).

Techniques: Concentration Assay, Ex Vivo, Organ Culture